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The bacteria are anaerobes. Aerobic and anaerobic microorganisms

Anaerobes and aerobes are two forms of existence of organisms on earth. The article deals with microorganisms.

Anaerobes are microorganisms that develop and multiply in an environment that does not contain free oxygen. Anaerobic microorganisms are found in almost all human tissues from purulent-inflammatory foci. They are classified as opportunistic (they exist in humans and develop only in people with a weakened immune system), but sometimes they can be pathogenic (disease-causing).

There are facultative and obligate anaerobes. Facultative anaerobes can develop and reproduce in both anoxic and oxygenic environments. These are microorganisms such as Escherichia coli, Yersinia, staphylococci, streptococci, Shigella and other bacteria. Obligate anaerobes can only exist in an oxygen-free environment and die when free oxygen appears in environment. Obligate anaerobes are divided into two groups:

  • bacteria that form spores, otherwise called clostridia
  • bacteria that do not form spores, or otherwise non-clostridial anaerobes.

Clostridia are causative agents of anaerobic clostridial infections - botulism, clostridial wound infections, tetanus. Non-clostridial anaerobes are the normal microflora of humans and animals. These include rod-shaped and spherical bacteria: bacteroides, fusobacteria, peillonella, peptococci, peptostreptococci, propionibacteria, eubacteria and others.

But non-clostridial anaerobes can significantly contribute to the development of purulent-inflammatory processes (peritonitis, abscesses of the lungs and brain, pneumonia, pleural empyema, phlegmon of the maxillofacial area, sepsis, otitis media and others). Most anaerobic infections caused by non-clostridial anaerobes are endogenous (internal origin, caused by internal causes) and develop mainly with a decrease in the body's resistance, resistance to the effects of pathogens as a result of injuries, operations, hypothermia, and decreased immunity.

The main part of anaerobes that play a role in the development of infections are bacteroides, fusobacteria, peptostreptococci and spore bacilli. Half of purulent-inflammatory anaerobic infections are caused by bacteroids.

  • Bacteroides are rods, 1-15 microns in size, motile or moving with the help of flagella. They secrete toxins that act as virulence (disease-causing) factors.
  • Fusobacteria are rod-shaped obligate (surviving only in the absence of oxygen) anaerobic bacteria that live on the mucous membrane of the mouth and intestines, can be immobile or motile, and contain a strong endotoxin.
  • Peptostreptococci are spherical bacteria, located in twos, fours, irregular clusters or chains. These are flagellate bacteria and do not form spores. Peptococci are a genus of spherical bacteria represented by one species, P. niger. Located singly, in pairs or in clusters. Peptococci do not have flagella and do not form spores.
  • Veyonella is a genus of diplococci (coccal-shaped bacteria, the cells of which are arranged in pairs), arranged in short chains, immobile, and do not form spores.
  • Other non-clostridial anaerobic bacteria that are isolated from infectious foci of patients are propionic bacteria, volinella, the role of which is less studied.

Clostridia is a genus of spore-forming anaerobic bacteria. Clostridia live on the mucous membranes of the gastrointestinal tract. Clostridia are mainly pathogenic (disease-causing) to humans. They secrete highly active toxins specific to each species. The causative agent of an anaerobic infection can be either one type of bacteria or several types of microorganisms: anaerobic-anaerobic (bacteroides and fusobacteria), anaerobic-aerobic (bacteroides and staphylococci, clostridia and staphylococci)

Aerobes are organisms that require free oxygen to survive and reproduce. Unlike anaerobes, aerobes have oxygen involved in the process of producing the energy they need. Aerobes include animals, plants and a significant part of microorganisms, among which are isolated.

  • obligate aerobes are “strict” or “unconditional” aerobes that receive energy only from oxidative reactions involving oxygen; these include, for example, some types of pseudomonads, many saprophytes, fungi, Diplococcus pneumoniae, diphtheria bacilli
  • In the group of obligate aerobes, microaerophiles can be distinguished - they require a low oxygen content to function. When hitting a normal external environment such microorganisms are suppressed or die because oxygen negatively affects the action of their enzymes. These include, for example, meningococci, streptococci, gonococci.
  • facultative aerobes are microorganisms that can develop in the absence of oxygen, for example, yeast bacillus. Most pathogenic microbes belong to this group.

For each aerobic microorganism there is a minimum, optimum and maximum oxygen concentration in its environment necessary for its normal development. An increase in oxygen content beyond the “maximum” limit leads to the death of microbes. All microorganisms die at an oxygen concentration of 40-50%.

Bacteria appeared more than 3.5 billion years ago and were the first living organisms on our planet. It was thanks to aerobic and anaerobic species of bacteria that life originated on Earth.

Today they are one of the most species-diverse and widespread groups of prokaryotic (nucleusless) organisms. Different respiration made it possible to subdivide them into aerobic and anaerobic, and nutrition into heterotrophic and autotrophic prokaryotes.

Classification division of prokaryotes

The species diversity of these anucleate, single-celled organisms is enormous: science has described only 10,000 species, but it is believed that there are more than a million species of bacteria. Their classification is extremely complex and is carried out based on the commonality of the following features and properties:

  • morphological – shape, mode of movement, ability to form spores, etc.);
  • physiological - breathing oxygen (aerobic) or oxygen-free version (anaerobic bacteria), by the nature of metabolic products and others;
  • biochemical;
  • similarity of genetic characteristics.

For example, morphological classification according to appearance classifies all bacteria as:

  • rod-shaped;
  • tortuous;
  • spherical.

The physiological classification in relation to oxygen divides all prokaryotes into:

  • anaerobic – microorganisms whose respiration does not require the presence of free oxygen;
  • aerobic - microorganisms that require oxygen for their vital functions.

Anaerobic prokaryotes

Anaerobic microorganisms fully correspond to their name - the prefix an- negates the meaning of the word, aero is air and b- life. It turns out - airless life, organisms whose breathing does not require free oxygen.

Anoxic microorganisms are divided into two groups:

  • facultative anaerobic – capable of existing both in an environment containing oxygen and in its absence;
  • obligate microorganisms - die in the presence of free oxygen in the environment.

The classification of anaerobic bacteria divides the obligate group according to the possibility of sporulation into the following:

  • spore-forming clostridia are gram-positive bacteria, most of which are motile, characterized by intense metabolism and great variability;
  • non-clostridial anaerobes are gram-positive and negative bacteria that are part of the human microflora.

Properties of clostridia

Spore-forming anaerobic bacteria are found in large numbers in the soil and in the gastrointestinal tract of animals and humans. Among them, more than 10 species are known that are toxic to humans. These bacteria produce highly active exotoxins that are specific to each species.

Although the infectious agent may be one type of anaerobic microorganism, intoxication by various microbial associations is more typical:

  • several types of anaerobic bacteria;
  • anaerobic and aerobic microorganisms (most often clostridia and staphylococci).

Bacterial culture

It is quite natural in the oxygen environment we are accustomed to that in order to obtain obligate aerobes it is necessary to use special equipment and microbiological media. In essence, the cultivation of oxygen-free microorganisms comes down to creating conditions under which the access of air to the environments where prokaryotes are cultivated is completely blocked.

In the case of microbiological analysis for obligate anaerobes, the methods of sampling and the method of transporting the sample to the laboratory are extremely important. Since obligate microorganisms will immediately die under the influence of air, the sample must be stored either in a sealed syringe or in specialized media intended for such transportation.

Aerophilic microorganisms

Aerobes are microorganisms whose respiration is impossible without free oxygen in the air, and their cultivation takes place on the surface of nutrient media.

According to the degree of dependence on oxygen, all aerobes are divided into:

  • obligate (aerophiles) - capable of developing only with a high concentration of oxygen in the air;
  • facultative aerobic microorganisms that develop even in low amounts of oxygen.

Properties and characteristics of aerobes

Aerobic bacteria live in soil, water and air and actively participate in the cycle of substances. The respiration of bacteria, which are aerobes, is carried out by direct oxidation of methane (CH 4), hydrogen (H 2), nitrogen (N 2), hydrogen sulfide (H 2 S), iron (Fe).

To obligate aerobic microorganisms, which are pathogenic for humans, include tuberculosis bacillus, tularemia pathogens and Vibrio cholerae. All of them require high levels of oxygen to function. Facultative aerobic bacteria, such as salmonella, are capable of respiration with very little oxygen.

Aerobic microorganisms that breathe in an oxygen atmosphere are able to exist in a very wide range at partial pressures from 0.1 to 20 atm.

Growing Aerobes

Cultivation of aerobes involves the use of a suitable nutrient medium. Necessary conditions are also quantitative control of the oxygen atmosphere and the creation of optimal temperatures.

The respiration and growth of aerobes manifests itself as the formation of turbidity in liquid media or, in the case of dense media, as the formation of colonies. On average, growing aerobes under thermostatic conditions will take about 18 to 24 hours.

General properties for aerobes and anaerobes

  1. All these prokaryotes do not have a pronounced nucleus.
  2. They reproduce either by budding or division.
  3. When carrying out respiration, as a result of the oxidative process, both aerobic and anaerobic organisms decompose huge masses of organic residues.
  4. Bacteria are the only living things whose respiration binds molecular nitrogen into an organic compound.
  5. Aerobic organisms and anaerobes are capable of respiration over a wide range of temperatures. There is a classification according to which nuclear-free single-celled organisms divided into:
  • psychrophilic – living conditions around 0°C;
  • mesophilic – temperature of vital activity from 20 to 40°C;
  • thermophilic - growth and respiration occurs at 50-75°C.

KINGDOM OF PROKARYOTES

SUB-KINGDOM REAL BACTERIA. SUBKINGDOM ARCHAEBACTERIA. SUBKINGDOM OXYPHOTOBACTERIA

Fill in the missing word.

1. Complete the sentences by inserting the necessary words.

All bacteria are grouped into the kingdom...

2. Science deals with the study of the structure and life activity of microorganisms - ...

3. A. Bacteria that exist in an oxygen-free environment are called...

B. Bacteria that exist in... the environment are called aerobes.

4. Cyanobacteria are often called...

5. Complete the sentence.

Cyanobacteria have played an important role in changing... the atmosphere, which is associated with their... activities.

6. In rice fields, nitrogen is used to enrich the soil...

7. Complete the sentences.

A. The most ancient bacteria on Earth are...

B. Methane-forming archaebacteria exist strictly in... conditions.

Choose the correct answer.

8. Method of movement of bacteria:

A. With the help of flagella

B. “Reactive” - throwing out mucus

B. Using wings

D. All statements are true

9. Establish the sequence of processes during bacterial reproduction.

A. Formation of daughter cells

B. The cell elongates

B. A transverse constriction is formed

D. Duplication of the bacterial chromosome

10. Establish the sequence of processes during sporulation.

A. Stopping metabolism in the cell

B. Separation of part of the cytoplasm containing hereditary material

B. Formation of a thick multilayer capsule

D. The cell becomes smaller in size

11. Bacterial spores are...

A. Sex cell

B. Propagation form

B. Form for the survival of bacteria in adverse conditions

D. Name of bacteria

12. To obtain energy, bacteria use:

A. Organic compounds

B. Inorganic compounds

B. Sunlight

D. All statements are true

Find a match.

13. Select the appropriate characteristics for bacteria of different types of nutrition:

I. Heterotrophs

II. Autotrophs of chemosynthesis

III. Autotrophs of photosynthesis

A. Formation of organic substances from inorganic ones due to the energy of sunlight

B. They feed on ready-made organic substances

B. Formation of organic substances from inorganic ones due to the energy of oxidation of inorganic substances

14. From the proposed terms, create a diagram of the biological cycle:

I. Organic matter

II. Not organic matter

III. Alive organisms

IV. Dead parts of living organisms and remains

V. Destroyer bacteria

Draw a conclusion about the role of destructive bacteria in the biological cycle.

15. Complete and draw up a diagram “The role of bacteria in human life.”

Using the diagram, write a story about the role of bacteria in human life.

16. Using the proposed terms, fill out the diagram:

I. Sulfur bacteria

II. Methanobacteria

III. Swamps, sewage treatment plants, animal rumen

IV. Methane gas

V. Sulfur and sulfur compounds

VI. Sulfuric acid

VII. Sulfur deposits

VIII. Archaebacteria

Using the diagram, prepare a story about the importance of archaeobacteria in nature.

17. Find the mistake in the sentence.

Based on their shape, bacteria are divided into cocci, bacilli, spirilla, thalli, and vibrio.

Choose the correct statement.

18. 1. Bacteria - diplococci are clusters of dense packs.

2. Flagella and villi are often developed on the surface of bacteria.

3. The cell wall of bacteria is formed by cellulose.

4. Bacteria lack membrane organelles, such as mitochondria and plastids.

5. In a bacterial cell, all enzymes that ensure its vital processes are located in the cytoplasm or on the inner surface of the cytoplasmic membrane.

6. Most bacteria are autotrophs.

7. Some bacteria synthesize organic matter from inorganic matter using the energy of oxidation of inorganic compounds.

8. Some bacteria can convert solar energy.

9. Bacteria destroy dead plant and animal organisms.

10. Bacteria convert organic substances into inorganic substances and return chemical elements into the biological cycle.

11. Bacteria only affect animals and humans.

12. The first bacteria appeared 7 billion years ago.

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Anaerobic bacteria are able to develop in the absence of free oxygen in the environment. Together with other microorganisms that have a similar unique property, they constitute the class of anaerobes. There are two types of anaerobes. Both facultative and obligate anaerobic bacteria can be found in almost all samples of pathological material; they accompany various purulent-inflammatory diseases, can be opportunistic and even sometimes pathogenic.

Anaerobic microorganisms, classified as facultative, exist and multiply in both oxygen and oxygen-free environments. The most pronounced representatives of this class are Escherichia coli, Shigella, staphylococci, Yersinia, streptococci and other bacteria.

Obligate microorganisms cannot exist in the presence of free oxygen and die from its exposure. The first group of anaerobes of this class is represented by spore-forming bacteria, or clostridia, and the second by bacteria that do not form spores (non-clostridial anaerobes). Clostridia are often causative agents of anaerobic infections of the same name. An example would be clostridial botulism and tetanus. Non-clostridial anaerobes are gram-positive and They have a rod-shaped or spherical shape; you have probably seen the names of their prominent representatives in the literature: bacteroides, veillonella, fusobacteria, peptococci, propionibacteria, peptostreptococci, eubacteria, etc.

Non-clostridial bacteria for the most part are representatives of the normal microflora in both humans and animals. They can also participate in the development of purulent-inflammatory processes. These include: peritonitis, pneumonia, abscess of the lungs and brain, sepsis, phlegmon of the maxillofacial area, otitis media, etc. The majority of infections that are caused by anaerobic bacteria of the non-clostridial type tend to exhibit endogenous properties. They develop mainly against the background of a decrease in the body's resistance, which can occur as a result of injury, cooling, surgery, or impaired immunity.

To explain the method of maintaining the vital functions of anaerobes, it is worth understanding the basic mechanisms by which aerobic and anaerobic respiration occurs.

Represents oxidative process, based on Breathing leads to the breakdown of the substrate without a residue, the result is split into energy-poor representatives of inorganics. The result is a powerful release of energy. Carbohydrates are the most important substrates for respiration, but both proteins and fats can be consumed in the process of aerobic respiration.

It corresponds to two stages of occurrence. At the first stage, an oxygen-free process of gradual breakdown of the substrate occurs to release hydrogen atoms and bind with coenzymes. The second, oxygen stage, is accompanied by further detachment from the substrate for respiration and its gradual oxidation.

Anaerobic respiration is used by anaerobic bacteria. They use not molecular oxygen, but a whole list of oxidized compounds to oxidize the respiratory substrate. They can be salts of sulfuric, nitric, and carbonic acids. During anaerobic respiration they are converted into reduced compounds.

Anaerobic bacteria that carry out such respiration as the final electron acceptor do not use oxygen, but inorganic substances. Based on their belonging to a certain class, several types of anaerobic respiration are distinguished: nitrate respiration and nitrification, sulfate and sulfur respiration, “iron” respiration, carbonate respiration, fumarate respiration.

The best solution for processing sewage waste in suburban conditions is to install a local treatment facility - a septic tank or biological treatment station.

The components that accelerate the decomposition of organic waste are bacteria for septic tanks - beneficial microorganisms that do not harm the environment. Agree, in order to correctly select the composition and dose of bioactivators, you need to understand the principle of their operation and know the rules for their use.

These issues are discussed in detail in the article. The information will help local sewerage owners improve the functioning of the septic tank and facilitate its maintenance.

Information about aerobes and anaerobes will be of interest to those who have decided for a suburban area or want to “modernize” an existing cesspool.

By selecting the right types of bacteria and determining the dosage (according to the instructions), you can improve the operation of the simplest storage type structure or establish the functioning of a more complex device - a two- or three-chamber septic tank.

Biological processing of organic matter is a natural process that has long been used by humans for economic purposes.

The simplest microorganisms, feeding on human waste, in a short period of time transform it into solid mineral sediment, clarified liquid and fat, which floats to the surface and forms a film.

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The use of bacteria for household and sanitary purposes is advisable for the following reasons:

  • Natural microorganisms that develop and live according to the laws of nature do not cause damage to the surrounding flora and fauna. This fact must be taken into account by owners of personal plots who use free territory for growing garden and vegetable crops, establishing lawns and flower beds.
  • There is no need to purchase aggressive chemicals, unlike natural elements that negatively affect the soil and plants.
  • The smell characteristic of household wastewater is felt much weaker or disappears altogether.
  • The cost of bioactivators is small compared to the benefits they bring.

Due to the pollution of soil and water bodies, the environmental problem has affected summer cottages, villages and territories with new suburban buildings - cottage villages. Thanks to the action of hygiene bacteria, it can be partially solved.

There are two types of bacteria involved in the sewage system: anaerobic and aerobic. More detailed information about the vital functions of two types of microorganisms will help you understand the principle of operation of septic tanks and storage tanks, as well as the nuances of maintaining treatment facilities.

Especially for servicing septic tanks and storage tanks, the production of household bioactivators has been launched, which are characterized by their low cost and simple preparation method (+)

Basic principles of anaerobic treatment

Microorganisms that can function in an oxygen-free environment are called anaerobic bacteria or anaerobes. Let's try to figure out what place they occupy in the wastewater treatment system.

Where do anaerobes come from?

It should be understood that the strains of anaerobic bacteria for household septic tanks were not specially bred artificially (although the production of new, more active strains has now been established); they have always been part of nature.

Microorganisms that can exist without oxygen develop in swampy and simply damp soil, in silt, and in soil at great depths. Some species actively participate in the formation of humus and humus, formed by the rotting of dead plants and dead animals.

Microorganisms that function in the soil and in water (in ponds, gutters, lakes) purify the liquid by separating minerals and gases. This ability was useful in the construction of sewer settling tanks and septic tanks

Anaerobic waste water is processed in sealed tanks. An example of such a container in a country house is an outdoor “birdhouse” toilet with. The fermentation process is carried out by anaerobic bacteria, which during their life processes produce methane and heat.

The anaerobic cleaning principle is also used in household devices (storage tanks, septic tanks) and industrial structures (digesters). Fermentation by anaerobes is actively used in livestock farms and poultry farms.

Conditions for the existence of bacteria

For the existence of microorganisms, special conditions are necessary, including:

  • isolation– lack of oxygen, with the exception of facultative anaerobes;
  • temperature regime– from +9ºС to +37ºС, optimal value – +28ºС;
  • pH value– acidity level from 6 to 8;
  • regularity of cleaning– removal of solid sediment.

As a result of the fermentation process, some substances sink to the bottom and rot, while others rise to the top. The liquid remains cloudy and often has a black tint. If a large amount of oxygen enters the tank, the bacteria can die.

Negative temperatures are also a primary threat, so it is recommended to insulate cesspools.

Anaerobic bacteria are an effective biological product for accelerating fermentation processes in storage cesspools. An example of a storage device is a summer-type country toilet.

For normal life, anaerobes need a liquid medium, that is, at least 2/3 of the container must be filled with water. If you do not call the vacuum cleaners in time, the amount of solid sediment will reach a critical level and the bacteria will begin to die.

If the storage capacity does not correspond to the number of residents, the container will have to be emptied quite often - 2-3 times a month. Therefore, the choice of a septic tank must be carefully considered, the parameters assessed and compared with the upcoming operating conditions.

Read more about selecting a treatment plant.

How does anaerobic treatment work?

The decomposition of organic matter in storage pits occurs in two stages. At first, you can observe sour fermentation, accompanied by a lot of unpleasant odor.

This is a slow process during which a primary sludge is formed that is marshy or gray in color and also emits a pungent odor. From time to time, pieces of silt break away from the walls and rise upward along with gas bubbles.

Over time, gases caused by acidification fill the entire volume of the container, displacing oxygen and creating an environment ideal for the development of anaerobic bacteria. From this moment, the alkaline decomposition of sewage begins - methane fermentation.

It has a completely different nature and, accordingly, different results. For example, the specific smell completely disappears, and the sludge takes on a very dark, almost black color.

VOC scheme using aerobes and anaerobes: two-chamber design designed for 95% wastewater treatment with additional treatment on the drainage field (+)

If a small part of alkaline sludge with anaerobes is placed in a storage tank or receiving chamber of a septic tank, the decomposition process will occur more efficiently, and the oxidation period will pass much faster.

In the absence of living sludge, it is necessary to purchase a bioactivator of suitable composition - a solution or dry substance in the form of tablets or powder, which is a complex of “dormant” anaerobic bacteria for the septic tank.

Thanks to anaerobes, the waste biomass in the cesspool quickly breaks down into solid sediment, gases and liquid, and is purified by approximately 65-70%.

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Advantages of anaerobic treatment:

  • small volume of bacterial biomass;
  • effective mineralization of organic matter;
  • lack of aeration, therefore saving on additional equipment;
  • possibility of using methane (in large quantities).

The disadvantages include strict adherence to living conditions: a certain temperature, pH value, regular removal of solid sediment. Unlike activated sludge, precipitated mineralized substances are not a nutrient medium for plants and are not used as fertilizer.

VOC schemes using anaerobic bacteria

The simplest device in which anaerobic bacteria can live and multiply is a drainage pit. Modern cesspools are concrete or installed in the ground below the freezing level.

HDPE products can be purchased from specialized companies or on manufacturers’ websites; concrete products can be purchased independently, with the help or under the supervision of specialists.

Diagram of the simplest septic tank, consisting of two compartments: in the first, combined (mechanical and anaerobic) cleaning is carried out, in the second - more anaerobic (+)

The productivity of the structure increases with the advent of additional functional chambers. The most common designs have 2-3 compartments, the first of which acts as a sump, and the next ones as tanks for cleaning and clarification.

To create a favorable environment for the life of microorganisms, porous material, for example, crushed stone, is placed at the bottom of the container.

Additional purification of the clarified liquid occurs in or on, through which water enters the soil. To install them, you need a suitable type of soil (loam, sand, sandy loam), as well as low level groundwater.

An example of installing a treatment facility, including an anaerobic septic tank and a filter well, in which wastewater is purified; The sand-crushed stone layer plays the role of a filter (+)

To maintain balance in the chambers, regular maintenance is necessary. It consists of removing solid sediment from the settling tank, pumping excess activated sludge from the second chamber to the first, adding bioactivators (in case of death or insufficient number of anaerobes).

Features of aerobic cleaning

Aerobes, unlike anaerobes, cannot exist without oxygen. They occupy their own, no less important, niche in the biological wastewater treatment system.

The conditions created for the development of anaerobes are absolutely unsuitable for the life of another type of bacteria - aerobes. They cannot form spores and grow in a confined space, deprived of air, and therefore process sewage.

For the aerobic system to reach its full potential, a vent is not enough; a forced supply of oxygen is necessary.

The air supply system for the aeration tank consists of several parts. The main function (air injection) is performed by. It is usually installed in a warm room inside the house, not far from the sewerage outlet. The air duct connects the compressor to the septic tank and runs in the same trench with pipes laid in the ground.

Diagram of a deep biological treatment station with three settling tanks and two aeration tanks with a capacity of 1.5 m³/day. Anaerobic and aerobic bacteria take part in wastewater purification (+)

Saturation of wastewater with oxygen occurs using perforated tubes lowered into the water - aerators. The air supply can be regulated or completely shut off by valves located near the hatch.

Air comes out of the holes and, moving upward in the form of small bubbles, enriches the drains with oxygen. Aerators need to be cleaned from time to time so that the oxygen supply process does not stop.

When installing the first VOC, the problem arose of the gradual flushing of aerobic bacteria for the septic tank. It was solved by loading additional devices into the container - textile shields and plastic “brush brushes”.

Fine-pile fabric and bristles made of polymer material prevent the washout of activated sludge and serve as a basis for its growth.

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As excess sludge accumulates, it is removed and used as fertilizer for growing vegetables, temporarily placed in compost heaps.

The main enemies of biological treatment are chemical detergents and antibiotics dissolved in sewage. They are destructive to various types of bacteria, so they are aggressive chemical substances(for example, chlorine and solutions containing it) are prohibited from being poured into a septic tank.

Advantages and disadvantages of using aerobes

Almost all existing deep biological treatment stations incorporate aerobic chambers, since “oxygen” bacteria have some advantages over anaerobes.

They destroy dissolved impurities in water remaining after mechanical and anaerobic treatment. In this case, no solid sediment is formed, and the plaque can be removed manually.

One of the options for installing a deep cleaning station with forced drainage into a ditch: for the operation of the compressor and drainage pump, a connection to the electrical network is required (+)

Activated sludge, which is the result of the activity of aerobes, is environmentally friendly and, unlike chemicals, benefits the vegetation growing on the site. Instead of the unpleasant odor characteristic of souring wastewater in cesspools, carbon dioxide comes out.

But the main advantage is the quality of water purification - up to 95-98%. The disadvantage is the energy dependence of the system.

In the absence of electrical power, the compressor stops supplying oxygen, and if left idle for a long time without aeration, bacteria can die. Both types of bacteria, aerobes and anaerobes, are sensitive to household chemicals, therefore, when using biological treatment, control of the composition of wastewater is necessary.

VOC schemes with aerobic treatment

The clarification of sewage wastewater with the help of aerobes is carried out in deep biological treatment stations. As a rule, such a station consists of 3-4 cameras.

The first compartment is a settling tank in which waste is divided into various substances, the second is used for anaerobic purification, and already in the 3rd (in some models and 4) compartment aerobic clarification of the liquid is carried out.

Installation diagram of a deep biological treatment station with an infiltrator and a storage well from which purified water is discharged into a ditch (+)

After three-four-stage treatment, the water is used for household needs (irrigation) or is supplied for additional treatment to one of the treatment facilities:

  • filter well;
  • filter field;
  • infiltrator.

But sometimes, instead of one of the structures, ground drainage is installed, in which post-treatment occurs in natural conditions. In sandy, gravel and crushed soils, the smallest remains of organic matter are processed by aerobes.

Through clays, loams, and almost all sandy loams except the sandy and highly fractured variants, water will not be able to seep into the underlying layers. Clay rocks also do not undergo ground purification, because... have extremely low filtration qualities.

If the geological section of the site is represented by clay soils, soil post-treatment systems (filtration fields, absorption wells, infiltrators) are not used.

An effective way to purify wastewater from a septic tank is a filtration field, which is a pit filled with gravel. Effluents come from the distribution well through drains, oxygen access is provided by risers

The filtration field is a branched system of perforated pipes (drains) extending from the distribution well. The treated wastewater flows first into the well, then into drains buried in the ground. The pipes are equipped with risers that supply oxygen needed by aerobic bacteria.

The infiltrator is a finished product made from HDPE, the last stage of VOCs for the post-treatment of clarified wastewater. It is buried in the ground next to the septic tank, placed on a drainage pad made of crushed stone. The conditions for installing the infiltrator are the same - light, permeable soil and a low groundwater level.

Installation of a group of infiltrators into the ground: to ensure the processing of a large volume of liquid and a higher degree of purification, several products are used connected by pipes

At first glance, a filter well resembles a storage tank, but has one significant difference - a penetrating bottom. The lower part remains open, covered with a 1-1.2 m drainage layer (crushed stone, gravel, sand). Ventilation and a technical hatch are required.

If additional purification is not required, purified to 95 - 98% wastewater directly from the septic tank into a roadside ditch or ditch.

Rules for the use of bioactivators

To start or enhance the biological treatment process, sometimes additives are needed - bioactivators in the form of dry powders, tablets or solutions.

They replaced bleach, which did more harm to the environment than good. For the production of bioactivators, the most persistent and active strains of bacteria living in the soil were selected.

When choosing a bioactivator, you should take into account such factors as the type of treatment plant, the location of the backfill, the specifics of the bacteria and enzymes included in the preparation

Drugs that help speed up the process of decomposition of organic matter usually have a universal complex composition, sometimes a narrowly targeted one. For example, there are starter varieties that help to “revive” the cleaning process after winter storage or long-term downtime.

Narrowly targeted types are aimed at solving a specific problem, for example, removing large amounts of grease from sewer pipes or breaking down concentrated soap effluents.

The use of bioactivators in VOCs and cesspools has a number of advantages.

Regular users note the following positive aspects:

  • reduction in the volume of solid waste by 65-70%;
  • destruction of pathogenic microflora;
  • disappearance of the pungent sewer smell;
  • faster cleaning process;
  • prevention of blockages and siltation various parts sewer system.

For rapid adaptation of bacteria, special conditions are required, for example, a sufficient amount of liquid in the container, the presence of a nutrient medium in the form of organic waste, or a comfortable temperature (on average from +5ºС to + 45ºС).

And do not forget that living bacteria in a septic tank are threatened by chemicals, petroleum products, and antibiotics.

An example of a universal type is the French bioactivator “Atmosbio”. Recommended for use in septic tanks, cesspools, country toilets. The cost of packaging is 300 g. – 600 rub.

The market for biological products does not experience a shortage; in addition to domestic brands, foreign ones are also widely represented. The most famous brands are “ Atmosbio", , "BioExpert", "Vodograi", , "Microzim Septi Treat", "Biosept".

Conclusions and useful video on the topic

The presented videos contain useful material on the selection and use of biological drugs.

Practical experience of using bioactivators in the village:

Microorganisms increase the efficiency of VOCs without causing harm to the environment. To create the most comfortable conditions for the life of bacteria, follow the instructions and do not forget to maintain the treatment facilities on time.

If you have anything to add, or have any questions on the topic of choosing and using bacteria for septic tanks, you can leave comments on the publication. The contact form is located in the lower block.